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Determination of Atropine Sulfate Using a Novel Sensitive DNA–Biosensor Based Electrochemical Interaction on Modified Pencil Graphite Electrode, Ali A. Ensafi, Parisa Nasr-Esfahani, Esmaeil Heydari-Bafrooei, B. Rezaei, Talanta, 2015, 131, 149-155.

A novel,selective,rapid and simple electrochemical method is developed for the determination of
atropine sulfate.UV–Vis and differential pulse voltammetry are used to study the interaction of atropine
sulfate with salmon sperm ds-DNA on the surface of salmon sperm ds-DNA modified-pencil graphite
electrode (PGE).For this purpose,a pencil graphite electrode (PGE) modified with multiwall carbon
nanotubes (MWCNTs), titanium dioxide nanoparticles (TiO2NPs), and poly-dialyldimethylammonium
chloride (PDDA) decorated with ds-DNA is tested for the determination of atropinesulfate.The
electrochemical oxidationpeakcurrentofadenineandguaninebondedonthesurfaceofds-DNA/
PDDA–TiO2NPs–MWCNTs/PGEisusedtoobtaintheanalyticalsignal.Decreasesintheintensitiesof
guanine andadenineoxidationsignalsaftertheirinteractionwithatropinesulfateareusedasindicator
signals forthesensitivedeterminationofatropinesulfate.Usingds-DNA/PDDA–TiO2NPs–MWCNTs/PGE
and basedontheguaninesignal,linearcalibrationcurveswereobtainedintherangeof0.6to
30.0 μmol L1 and 30.0to600.0 μmol L1 atropine sulfatewithlowdetectionlimitsof30.0nmolL1.
The biosensorshowsagoodselectivityforthedeterminationofatropinesulfate.Finally,theapplicability
of thebiosensorisevaluatedbymeasuringatropinesulfateinrealsampleswithgoodaccuracy.

Journal Papers
Month/Season: 
January
Year: 
2015