Fabrication of an Electrochemical DNA Biosensor for Bacillus Cereus Detection in Milk and Infant Formula, 366. Z. Izadi, M. Shekhzeynoddin, Ali A. Ensafi, Biosensors & Bioelectronics, 2016, 80, 582-589.
This paper describes fabrication of a DNA-based Au-nanoparticle modiﬁed pencil graphite electrode
(PGE) biosensor for detection of Bacillus cereus, causative agent of two types of food-borne disease, i.e.,
emetic and diarrheal syndrome. The sensing element of the biosensor was comprised of gold nano-
particles (GNPs) self-assembled with single-stranded DNA (ssDNA) of nheA gene immobilized with thiol
linker on the GNPs modiﬁed PGE. The size, shape and dispersion of the GNPs were characterized by ﬁeld
emission scanning electron microscope (FESEM). Detection of B. cereus was carried out based on an
increase in the charge transfer resistance (R ct ) of the biosensor due to hybridization of the ss-DNA with
target DNA. An Atomic force microscope (AFM) was used to conﬁrm the hybridization. The biosensor
sensitivity in pure cultures of B. cereus was found to be 10 0 colony forming units per milliliter (CFU/mL)
with a detection limit of 9.4 10 12 mol L 1 . The biosensor could distinguish complementary from
mismatch DNA sequence. The proposed biosensor exhibited a rapid detection, low cost, high sensitivity
to bacterial contamination and could exclusively and speciﬁcally detect the target DNA sequence of B.
cereus from other bacteria that can be found in dairy products. Moreover, the DNA biosensor exhibited
high reproducibility and stability, thus it may be used as a suitable biosensor to detect B. cereus and to
become a portable system for food quality control.