A novel,selective,rapid and simple electrochemical method is developed for the determination of
atropine sulfate.UV–Vis and differential pulse voltammetry are used to study the interaction of atropine
sulfate with salmon sperm ds-DNA on the surface of salmon sperm ds-DNA modified-pencil graphite
electrode (PGE).For this purpose,a pencil graphite electrode (PGE) modified with multiwall carbon
nanotubes (MWCNTs), titanium dioxide nanoparticles (TiO2NPs), and poly-dialyldimethylammonium
chloride (PDDA) decorated with ds-DNA is tested for the determination of atropinesulfate.The
electrochemical oxidationpeakcurrentofadenineandguaninebondedonthesurfaceofds-DNA/
PDDA–TiO2NPs–MWCNTs/PGEisusedtoobtaintheanalyticalsignal.Decreasesintheintensitiesof
guanine andadenineoxidationsignalsaftertheirinteractionwithatropinesulfateareusedasindicator
signals forthesensitivedeterminationofatropinesulfate.Usingds-DNA/PDDA–TiO2NPs–MWCNTs/PGE
and basedontheguaninesignal,linearcalibrationcurveswereobtainedintherangeof0.6to
30.0 μmol L
1 and 30.0to600.0 μmol L
1 atropine sulfatewithlowdetectionlimitsof30.0nmolL
1.
The biosensorshowsagoodselectivityforthedeterminationofatropinesulfate.Finally,theapplicability
of thebiosensorisevaluatedbymeasuringatropinesulfateinrealsampleswithgoodaccuracy.