Authors: Leila Mirmoghtadaie, Ali A Ensafi, Mahdi Kadivar, Parviz Norouzi

Publication date: 2013/4/1

Journal name: Materials Science and Engineering: C

Volume: 33

Pages: 1753-1758

Publisher: Elsevier

An electrochemical DNA biosensor was proposed as a screening device for the rapid
analysis of folic acid using a pencil graphite electrode modified with salmon sperm ds-DNA.
At first, immobilization of the ds-DNA on pencil graphite electrode was optimized using
response surface methodology. Solution pH, DNA concentration, time of DNA deposition
and potential of deposition was optimized each at three levels. The optimum combinations
for the reaction were pH4. 8, DNA concentration of 24μgmL− 1, deposition time of 304s, ...