Highly selective flow-injection spectrophotometric determination of ascorbic acid in fruit juices and pharmaceuticals using pyrogallol red-iodate system

Authors Ali A Ensafi, B Rezaei, M Beglari

Publication date: 2002/5/28

Journal name: Analytical letters

Volume: 35

Pages: 909-920

Publisher: Taylor & Francis Group

ABSTRACT Ascorbic acid present in pharmaceuticals and fruit juice was rapidly determined
by the spectrophotometric method in association with flow injection analysis. The method is
based on the inhibitory effect of ascorbic acid on the oxidation of pyrogallol red by iodate in
sulfuric acid media. The calibration graph is linear in the range of 2.0× 10− 6 M up to 1.0×
10− 4 M. The throughput is 20× 5 samples per hour and the detection limit is 0.5 µM ascorbic
acid. The relative standard deviation for ten replicate determinations of 8.00 µM and 50.0 ...