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Flow-injection determination of ascorbic acid and cysteine simultaneously with spectrofluorometric detection

Authors: Behzad Rezaei, Ali Asghar Ensafi, Siavash Nouroozi
Publication date: 2005
Journal name: Analytical sciences
Volume: 21
Pages: 1067-1071
Publisher: 社団法人日本分析化学会
 
A simple and sensitive spectrofluorometric method was developed for the simultaneous
determination of ascorbic acid and cysteine by a flow-injection system. This method is based
on the reduction of Tl (III) with ascorbic acid or cysteine in acidic media, producing
fluorescence reagent, TlCl 3 2-(λ ex= 227 nm, λ em= 419 nm). The injected sample solution
was divided into two separate streams. The first stream was treated with Tl (III) at pH 3.0 and
then passed through a 270 cm reaction coil to the flow cell of the spectrofluorometer, ...

http://www.jstage.jst.go.jp/article/analsci/21/9/21_9_1067/_article

Journal Papers
Month/Season: 
January
Year: 
2005

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