Ali A. Ensafi, Fatemeh Mirzaii, Parisa Nasr-Esfahani, Behzad Rezaei, Electroanalysis,

Abstract: In this work, an electrochemical sensor based on Ni3S2 nanoparticles supported on porous ball-milled silicon was fabricated for measuring glucose. At first, the glassy carbon electrode (GCE) surface was modified by Ni3S2 nanoparticles supported on a porous ball-milled silicon substrate. To characterize the modified electrode, N2 adsorption-desorption isotherms and BHJ, transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), elemental mapping and X-ray diffraction (XRD) were used. In the following, the effective parameters on the sensor response such as pH, NaOH concentration, catalyst concentration, applied potential, and rotational speed of the electrode were optimized using cyclic voltammetric (CV) and hydrodynamic amperometric methods. Under the optimal conditions, the calibration curve was plotted using the hydrodynamic amperometric method. Three linear regions were obtained from 0.5–134, 134–1246, and 1246–3546 μM, with a detection limit of 0.2 μM for glucose. Finally, the proposed method was used for measuring glucose levels in human blood serums.

Keywords: Electrochemical sensor · Modified glassy carbon electrode · Porous silicon · Ni3S2 · Glucose