A theoretical and experimental study of polyaniline/GCE and DNA G-quadruplex conformation as an impedimetric biosensor for the determination of potassium ion

An electrochemical aptasensor has been developed to determine K⁺ using electrochemical impedance spectroscopy. The polyaniline (PANI) coating was first electrodeposited on a GCE. Then, the potassium-selective aptamer [G₃(T₂AG₃)₃] was adsorbed through an electrostatic force between PANI and aptamer. In the presence of K⁺, the single-stranded DNA is folded into the G-quadruplex configuration, which acts as a barrier against electron transfer at the GCE surface. AFM and FE-SEM images characterize the surface morphology at each fabrication stage. As the K⁺ concentration increased, the charge transfer resistance (R_ct) increased, and the plot of ΔR_ct versus the logarithm of the K⁺ concentration is linear over a wide range of 10 pM–60 μM with a low detection limit of 3.7 pM. Finally, the proposed sensor was used to determine K⁺ in water, serum, urine, and fruit samples. Moreover, the binding stability of the aptamer/PANI and K⁺/Aptamer/PANI and the interactions between the aptamer and PANI were analyzed through molecular dynamics simulation.

Keywords: Potassium ion Biosensor Polyaniline Aptamer Electrochemical impedance spectroscopy Molecular dynamics simulation